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1.
Materials (Basel) ; 17(4)2024 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-38399061

RESUMO

This work develops a three-dimensional (3D) weak formulation, based on the consistent couple stress theory (CCST), for analyzing the size-dependent dynamic instability behavior of simply-supported, functionally graded (FG) cylindrical microshells that are subjected to combinations of periodic axial compression and external pressure. In our formulation, the microshells are artificially divided into nl layers. The displacement components of each individual layer are selected as the primary variables, which are expanded as a double Fourier series in the in-plane domain and are interpolated with Hermitian C2 polynomials in the thickness direction. Incorporating the layer-wise displacement models into our weak formulation, we develop a Hermitian C2 finite layer method (FLM) for addressing the current issue. The accuracy and the convergence rate of our Hermitian C2 FLM are validated by comparing the solutions it produces with the accurate two-dimensional solutions of critical loads and critical pressures of FG cylindrical macroshells and single-walled carbon nanotubes, which were reported in the literature. The numerical results show the effects of the material length-scale parameter, the inhomogeneity index, the radius-to-thickness and length-to-radius ratios, the load magnitude ratio, and the static and dynamic load factors on the first principal and first secondary instability regions of parametric resonance of simply-supported FG cylindrical microshells are significant.

2.
Clin Oral Investig ; 28(1): 114, 2024 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-38267793

RESUMO

OBJECTIVES: Among the existing techniques for the correction of mandibular posterior vertical insufficiency (PVI), the intra-oral ramus vertical lengthening osteotomy (IORVLO) can be proposed as it allows simultaneous correction of mandibular height and retrusion. This study assessed the 3D morpho-anatomical changes of the ramus-condyle unit and occlusal stability after IORVLO. MATERIALS AND METHODS: This retrospective analysis compared immediate and 1-year post-operative 3D CBCT reconstructions. The analysis focused on the condylar height (primary endpoint) and on the changes in condylar (condylar diameter, condylar axis angle) and mandibular (ramus height, Frankfort-mandibular plane angle, gonion position, intergonial distance, angular remodeling) parameters. Additionally, this analysis investigated the maxillary markers and occlusal stability. RESULTS: On the 38 condyles studied in 21 included patients (mean age 23.7 ± 3.9 years), a condylar height (CH) loss of 0.66 mm (p < 0,03) was observed, with no correlation with the degree of ramus lengthening (mean 13.3 ± 0.76 mm). Only one patient presented an occlusal relapse of Class II, but a 3.4 mm (28%) condylar diameter loss and a 33% condylar volume reduction with loss of 1 mm and 3.4 mm in CH and condyle diameter, respectively. A mean 3.56 mm (p < 0.001) decrease in ramus height was noted, mainly due to bone resorption in the mandibular angles. CONCLUSION: This study confirms the overall stability obtained with IORVLO for the correction of PVI. CLINICAL RELEVANCE: This study aims to precise indication of IORVLO, and to validate the clinical and anatomical stability of results.


Assuntos
Reabsorção Óssea , Procedimentos de Cirurgia Plástica , Humanos , Adulto Jovem , Adulto , Estudos Retrospectivos , Osteotomia , Mandíbula/diagnóstico por imagem , Mandíbula/cirurgia , Polímeros
3.
Acta Otolaryngol ; 143(11-12): 931-935, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-38127466

RESUMO

Background: The cochlear aqueduct (CA), which connects the scala tympani and the subarachnoid space, and its accompanying structures appear to have a significant relevance during cochlear implantation and an accurate visualization in clinical imaging is of great interest. Aims and Objective: This study aims to determine which potential and limitations clinically available imaging modalities have in the visualization of the CA. Methods: Micro-CT, flat-panel volume computed tomography with and without secondary reconstruction (fpVCT, fpVCTseco) and multislice computed tomography (MSCT) of 10 temporal bone specimen were used for 3D analysis of the CA. Results: FpVCTseco proved superior in visualizing the associated structures and lateral portions of the CA, which merge into the basal turn of the cochlea. All clinical imaging modalities proved equal in analyzing the length, total volume of the CA and its area of the medial orifice. Conclusion: The choice of the most accurate clinical imaging modality to evaluate the CA and its associated structures depends on the clinical or scientific question. Furthermore, this study should provide a basis for further investigations analyzing the CA.


Assuntos
Implante Coclear , Implantes Cocleares , Aqueduto da Cóclea/diagnóstico por imagem , Aqueduto da Cóclea/cirurgia , Cóclea/diagnóstico por imagem , Cóclea/cirurgia , Implante Coclear/métodos , Osso Temporal/cirurgia , Microtomografia por Raio-X
4.
Angle Orthod ; 93(6): 667-674, 2023 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-37922391

RESUMO

OBJECTIVES: To investigate three-dimensional (3D) morphologic changes in the alveolar bone around the maxillary central incisors of patients who underwent premolar extraction and subsequent anterior tooth retraction using temporary anchorage devices (TADs). MATERIALS AND METHODS: The subjects consisted of 16 patients with bimaxillary protrusion. The maxillary anterior teeth were retracted using sliding or loop mechanics and TADs for anchorage reinforcement. Cephalograms and computed tomography scans taken pretreatment and posttreatment were registered with respect to the palatal structures. The movement of the maxillary central incisors and morphologic changes in the anterior alveolar bone were evaluated quantitatively. RESULTS: Displacement in the palatal direction was observed in the alveolar bone around the incisors and the interdental septum. The displacement and bone remodeling/tooth movement ratio were larger on the labial side than the palatal side, and decreased progressively from the crest to apex level. The bone thickness was significantly increased on the labial side and decreased on the palatal side. CONCLUSIONS: Regional differences exist in morphologic changes of the alveolar bone during anterior tooth retraction using TADs. Attention should be paid to the crest region of the palatal alveolar bone because of its small original thickness and low remodeling activity.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Tomografia Computadorizada por Raios X , Humanos , Assistência Odontológica , Incisivo/diagnóstico por imagem , Maxila/diagnóstico por imagem , Técnicas de Movimentação Dentária
5.
Clin Oral Investig ; 27(12): 7489-7499, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37971540

RESUMO

OBJECTIVES: The aim was to compare the porosity of different bulk-fill resin-based composites (RBCs) placement techniques to the conventional incremental technique using microcomputed tomography (µ-CT). MATERIAL AND METHODS: Occlusal cavities were prepared on extracted human molars, divided into five groups based on the placement technique (n = 10/group). Techniques examined were Monoblock-two-step (SureFil SDR flow + Ceram.X), Monoblock-two-step (Tetric EvoFlow Bulk-Fill + Tetric EvoCeram Bulk-Fill), Monoblock-one-step (Tetric EvoCeram Bulk-Fill), Monoblock with sonic activation (SonicFill2), and incremental technique (Filtek Z250). µ-CT scanning (SkyScan, Bruker, Belgium) assessed the number, volume of closed pores, and total porosity. Analysis of variance on ranks was used (Student-Newman-Keuls method and Mann-Whitney rank-sum test), to determine the significance of RBC viscosity and the sonication placement technique. The Spearman correlation method assessed the correlation between porosity characteristics (α = 0.05). RESULTS: The SonicFill2 presented a higher number of closed pores than the other groups (p < 0.05). The overall porosity within the restoration seemed greater in this order: Filtek Z250 > SonicFill2 > Tetric EvoFlow Bulk-Fill + Tetric EvoCeram Bulk-Fill > Tetric EvoCeram Bulk-Fill > SureFil SDR Flow + Ceram.X. Sonication was associated with increased number (p = 0.005) and volume (p = 0.036) of closed pores. A strong correlation was observed between the number and volume of closed pores (R2 = 0.549, p < 001). CONCLUSIONS: The monoblock technique with sonic activation showed significantly more internal porosity than the other placement techniques. Sonication during application contributed to the higher number and volume of closed pores than the passive bulk-fill application. CLINICAL RELEVANCE: Using bulk-fill materials enhances efficiency, yet void formation remains an issue, depending on viscosity and active/passive delivery of materials. Clinicians must familiarize themselves with effective placement techniques to reduce void formation and optimizing treatment outcomes.


Assuntos
Resinas Compostas , Materiais Dentários , Humanos , Microtomografia por Raio-X , Porosidade , Teste de Materiais , Viscosidade , Restauração Dentária Permanente/métodos
6.
Stomatologiia (Mosk) ; 102(6): 33-38, 2023.
Artigo em Russo | MEDLINE | ID: mdl-37997311

RESUMO

OBJECTIVE: To assess the prevalence and quantitative characteristics of skeletal asymmetries of the body and lower jaw branches in three-dimensional space. MATERIALS AND METHODS: Using depersonalized data archive, of 400 CBCT scans, forty studies were picked randomly. Patients aged 25 to 35 years participated in research, no history of maxillofacial reconstructive surgery; gender, race, and facial anomaly were ignored. Three experienced doctors, independently, identified 15 reference points on radiographic images of AUTOPLAN software. Expert consensus found in the orientation determination. Six planes and eight points were constructed, using Math algorithms and the Python OOP; body and branches of the lower jaw length measurements performed and processed automatically. RESULTS: The study identified symmetry, using a method for determining parameters of the lower jaw in 3D space (Priority Reference No.2023100466 at 10.01.2023), modern dentistry research method. Both sides of data obtained shown asymmetry of the body and branches of the lower jaw occurred in 45% (18 people) and 67.5% (27 people) of cases. Forty percent of cases were noted with Mild asymmetry of the lower jaw body (16 people), moderate asymmetry noted in 5% (2 people). These parameters were 57.5% for branches, (23 people) and 10% (4 people). Combined form of anomaly observed in 37.5% (15 people) of cases, among which 12 people (75%) showed adaptive compensation of anomalies, which requires further investigation on a larger number of patients. CONCLUSION: Lower body jaw asymmetry is prevalent (80%). Patients with moderate and serious anomaly form appeared in 15% of cases.


Assuntos
Assimetria Facial , Mandíbula , Humanos , Cefalometria/métodos , Mandíbula/diagnóstico por imagem , Mandíbula/anormalidades , Tomografia Computadorizada de Feixe Cônico/métodos , Software , Imageamento Tridimensional/métodos
7.
Front Pediatr ; 11: 1291739, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37954430

RESUMO

Objectives: Recurrent patellar dislocation (RPD) greatly affects active young individuals, necessitating the identification of risk factors for a better understanding of its cause. Previous research has connected RPD to lower limb alignment (LEA) abnormalities, such as increased femoral anteversion, tibial external rotation, knee valgus, and flexion. This study aims to use EOS technology to detect RPD-related LEA anomalies, enabling three-dimensional assessment under load conditions. Methods: A total of 100 limbs (50 in the RPD group, 50 in the control group) were retrospectively analyzed. In the RPD group, we included limbs with recurrent patellar dislocation, characterized by dislocations occurs at least two times, while healthy limbs served as the control group. We used EOS technology, including 2D and 3D imaging, to measure and compare the following parameters between the two groups in a standing position: Femoral neck shaft angle (NSA), Mechanical femoral tibial angle (MFTA), Mechanical lateral distal femoral angle (mLDFA), Medial proximal tibial angle (MPTA), Anatomical femoral anteversion (AFA), External tibial torsion (ETT), and Femorotibial rotation (FTR). Results: The significant differences between the two groups were shown in NSA 3/2D, MFTA 3/2D, mLDFA 3/2D, MPTA 3D, AFA, FTR. No significant difference was shown in MPTA 2D, ETT between the RPD group and the control group. Further binary logistic regression analysis. Further binary logistic regression analysis was conducted on the risk factors affecting RPD mentioned above. and found four risk factors for binary logistic regression analysis: mLDFA (3D), AFA, NSA(3D), and FTR. Conclusions: EOS imaging identified abnormal LEA parameters, including NSA, MFTA, mLDFA, MPTA, AFA, and FTR, as risk factors for RPD. Children with these risk factors should receive moderate knee joint protection.

8.
Cureus ; 15(9): e45208, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37842472

RESUMO

Background and objective The complex structural integrity of the orbit is crucial for visual functions. Three-dimensional (3D) reconstructions from radiological images have revolutionized anatomical understanding, eliminating the limitations of two-dimensional (2D) imaging and offering intricate spatial details for complex structures. The aim of this study was to analyze the detailed morphometry of the orbit in healthy Anatolian adults, considering sex, side discrepancies, and clinical relevance using 3D models reconstructed from multidetector computed tomography (MDCT) images. Materials and methods Fifty-six (44.44%) males and 70 (55.56%) females (total: 126, mean age: 48.62) MDCT images were randomly selected and 3D skull models were reconstructed using 3D Slicer software. Measurements were conducted in millimeters (mm) for transverse and vertical diameters, circumference of the orbital opening, orbital wall lengths, as well as intraorbital and extraorbital distances. Results The method of measurements showed high reproducibility of results. The mean values for transverse and vertical diameters, circumference of the orbital opening, medial and lateral wall lengths, roof and floor lengths, and intraorbital and extraorbital distances were 40.23±2.12 mm (p<0.001), 34.94±2.16 mm (p=0.365), 44.74 ±3.02 mm (p<0.001), 46.30±2.69 mm (p<0.001), 51.26±2.91 mm (p<0.001), 49.01±3.22 mm (p<0.001), 126.10±5.71 mm (p<0.001), 19.63±2.35 mm (p=0.026), and 94.09±4.84 mm (p<0.001), respectively. Conclusion The study's high measurement reproducibility contributes significantly to the existing literature and clinical practice. These findings offer specific insights into Anatolian orbital morphometry, aiding in surgical planning, implant placement, and diagnostic assessments. The precise measurement values serve as a reliable reference for clinicians, facilitating the identification of normal and abnormal orbital anatomy and enhancing patient care. We believe this study provides valuable data for craniofacial and ophthalmological research, benefiting both clinical practice and future research endeavors in these fields.

9.
J Sports Sci Med ; 22(3): 571-581, 2023 09.
Artigo em Inglês | MEDLINE | ID: mdl-37711705

RESUMO

The objective of this research was to compare technical skills composed of kinematic and kinetic variables in the complex motor task of a tumble turn between 9 elites and 9 sub-elite female swimmers. The best tumble turn among three attempts was analyzed using a three-dimensional underwater protocol. A total of 37 kinematic variables were derived from a Direct Linear Transformation algorithm for 3D reconstruction, and 16 kinetic variables measured by a piezoelectric 3D force platform. Data were analyzed by Student's t-test and effect size statistics. Pearson correlations were applied to the data of the eighteen swimmers to relate the association of 53 kinematic, kinetic variables to the performance of the tumble-turn (3 meters Round Trip Time, 3m RTT). The approach and the whole turn times were faster for elite swimmers compared to sub elites (1.09±0.06 vs. 1.23±0.08 sec, and 2.89±0.07 vs. 3.15±0.11 sec.), as well as the horizontal speeds of the swimmers' head 1 m before the rotation (1.73±0.13 vs. 1.57±0.13 m/sec.), at the end of the push-off on force platform (2.55±0.15 vs. 2.31±0.22 m/sec.) and 3 m after the wall (2.01±0.19 vs. 1.68±0.12 m/sec.). Large differences (|d| > 0.8) in favor of the elite swimmers were identified for the index of upper body extension at the beginning of the push-off, the lower limb extension index at the end of push-off, and among the kinetic variables, the horizontal impulse and lateralization of the push-off. Correlations for the whole group revealed a moderate to strong relationship between 6 body extension indices and 3mRTT performance. For the kinetic variables, the correlations indicated the fastest swimmers in 3mRTT showed large lateral impulse during placement (r=0.46), maximum horizontal force during the push-off (r=0.45) and lateralization of the push-off (r=0.44) (all p<0.05). Elite female swimmers had higher approach and push-off speeds, were more streamlined through the contact, and showed a higher horizontal impulse and lateralization of the push-off, than their sub-elite counterparts.


Assuntos
Algoritmos , Extremidade Inferior , Humanos , Feminino , Cinética , Rotação
10.
Ear Nose Throat J ; : 1455613231198982, 2023 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-37752821

RESUMO

Object: Three-stage expansion method represents the most common form of microtia reconstruction in hemifacial microsomia (HFM). Although the complication related expander has lowered owing to the current advances, bony depression in mastoid region in microtia patients with hemifacial microsomia was observed in clinical work. The aim of this study was to quantify bony depression after retroauricular expander implantation and identify associated factors. Methods: 42 patients were enrolled and studied prospectively utilizing 3-dimensional (3D) evaluation. Craniofacial computed tomography (CT) was performed before the first (pre-expansion) and the second stage (post-expansion) and 3D quantification was done to quantify bony depression in mastoid region by using CT data. Univariate analysis was performed to identify factors associated with bony depression in mastoid region. Results: The mean level of mastoid depression was 0.83 mm (range: 0.07-4.08 mm), and the max level of mastoid depression was 1.40 mm (range: 0.20-6.65 mm). In univariate analysis, capsular duration of expansion and expansion volume were associated factors with mastoid depression. Conclusion: This study showed the possibility of mastoid depression following expander implantation for microtia reconstruction in hemifacial microsomia. Plastic surgeons should be aware of the possibility and associated factors of bony depression in mastoid region following expander implantation to optimize microtia reconstruction for patients with HFM.

11.
Ear Nose Throat J ; : 1455613231197768, 2023 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-37735888

RESUMO

Objective: Microtia patients with hemifacial microsomia (HFM) have a host of distinct anatomical disorder of skeletal and soft tissue asymmetries. The purpose of this study was to assess soft tissue discrepancies in microtia patients with HFM and their correlation with skeletal discrepancies. Methods: A total of 42 patients were enrolled and studied prospectively using a 3-dimensional superimposition and color mapping of the soft and hard tissues. Mirroring techniques created perfectly symmetric models for comparison. Differences between affected and normal sides were evaluated in 5 areas: retroauricular mastoid, malar, maxillary frontal, mandibular frontal, and gonion areas. Pearson correlations were used to assess the relationship between skeletal and soft tissue asymmetry. Results: Hard tissue asymmetry ranged from 0.79 mm (mandibular frontal) to 1.29 mm (malar), while soft tissue asymmetry ranged from 1.34 mm (maxillary frontal) to 5.26 mm (retroauricular mastoid). Correlations between skeletal and soft tissue asymmetry varied, with the strongest correlation observed at the retroauricular mastoid area and the weakest at the maxillary frontal area. Conclusion: There was a high correlation between bone and soft tissue hypoplasia at the retroauricular mastoid area, while the other evaluated areas showed poor correlation between skeletal and soft tissue asymmetries. Clinicians should assess each component separately for optimal treatment planning in microtia patients with HFM.

12.
Eur Spine J ; 32(11): 4020-4029, 2023 11.
Artigo em Inglês | MEDLINE | ID: mdl-37747546

RESUMO

INTRODUCTION: Adult degenerative scoliosis (ADS) is a 3D deformity that greatly affects the quality of life of patients and is closely related to the quality of paraspinal muscles (PSMs), but the specific degenerative characteristics have not been described. METHODS: This study included ADS patients who were first diagnosed in our hospital from 2018 to 2022. Muscle volume (MV) and fat infiltration (FI) of PSM were measured by 3D reconstruction, and spinal parameters were assessed by X-ray. The values of convex side (CV) and concave side (CC) were compared. RESULTS: Fifty patients were enrolled with a mean age of 64.1 ± 5.8 years old. There were significant differences in MV, FI, and Cobb angle between male and female groups. The MV of MF and PS on the CC was significantly larger than that on the CV. In the apex and the segments above the apex, the FI of the MF on the CC is greater than the CV, and in the CV of the segment below the apex, the FI of the MF is greater than the CC. Besides, there was a significant positive correlation between the FI and Cobb angle in the MF of the CC-CV. CONCLUSION: There were significant differences in the MV and FI of PSM on both sides of the spine in ADS patients. It was determined that the PSM of ADS showed different degrees of degeneration in different levels of the lumbar spine and were positively correlated with Cobb angle.


Assuntos
Escoliose , Humanos , Masculino , Adulto , Feminino , Pessoa de Meia-Idade , Idoso , Escoliose/complicações , Escoliose/diagnóstico por imagem , Escoliose/patologia , Músculos Paraespinais/diagnóstico por imagem , Músculos Paraespinais/patologia , Qualidade de Vida , Radiografia , Vértebras Lombares/diagnóstico por imagem , Atrofia Muscular/diagnóstico por imagem
13.
Sensors (Basel) ; 23(15)2023 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-37571537

RESUMO

Corals play a crucial role as the primary habitat-building organisms within reef ecosystems, forming expansive structures that extend over vast distances, akin to the way tall buildings define a city's skyline. However, coral reefs are vulnerable to damage and destruction due to their inherent fragility and exposure to various threats, including the impacts of climate change. Similar to successful city management, the utilization of advanced underwater videography, photogrammetric computer vision, and machine learning can facilitate precise 3D modeling and the semantic mapping of coral reefs, aiding in their careful management and conservation to ensure their survival. This study focuses on generating detailed 3D mesh models, digital surface models, and orthomosaics of coral habitats by utilizing underwater coral images and control points. Furthermore, an innovative multi-modal deep neural network is designed to perform the pixel-wise semantic segmentation of orthomosaics, enabling the projection of resulting semantic maps onto a 3D space. Notably, this study achieves a significant milestone by accomplishing semantic fine-grained 3D modeling and rugosity evaluation of coral reefs with millimeter-level accuracy, providing a potent means to understand coral reef variations under climate change with high spatial and temporal resolution.


Assuntos
Antozoários , Recifes de Corais , Animais , Ecossistema , Semântica , Mudança Climática , Aprendizado de Máquina
14.
Prog Orthod ; 24(1): 25, 2023 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-37455279

RESUMO

BACKGROUND: Maxillary molar distalization is a common technique used in the non-extraction treatment of Angle Class II malocclusion that can effectively correct the molar relationship and create spaces for anterior teeth alignment. However, this approach may also impact the temporomandibular joint (TMJ) due to predictable changes in the posterior vertical dimension. Despite its widespread use, Class II malocclusions correction by molar distalization with clear aligners has not been investigated for their effects on the TMJ. Therefore, this study aimed to analyze the impact of sequential molar distalization using clear aligners on the TMJ. METHODS: Three-dimensional CBCT scans of 23 non-growing patients (7 males, 16 females; mean age 29.8 ± 4.6 years) with skeletal class I or II malocclusion and a bilateral molar class II relationship treated by sequential upper molars distalization with orthodontic clear aligners (Invisalign, Align Technology, San Josè, Ca, USA). A total of 46 joints were examined before and after molar distalization using Anatomage InvivoDental 6.0.3. Linear and angular measurements of the mandibular joint were measured, including joint parameters, inclination, position, and the dimension of the condyle and articular fossa. In addition, 3D volumetric spaces of the joint were analyzed. All data were statistically analyzed by paired T test to determine the differences between the pre-and post-orthodontic procedures. RESULTS: No statistically significant differences were found in all primary effects resulting from maxillary molars distalization by clear aligners on TMJ components measurements and joint spaces between T0 and T1. Meanwhile, statistically significant differences were observed in the linear position of the upper molars and the molar relationship parameter with at least P ≤ 0.05. CONCLUSION: Treatment by sequential upper molars distalization with clear aligners does not lead to significant TMJ parameters changes in condyle and fossa spaces, dimensions, and positions.


Assuntos
Má Oclusão Classe II de Angle , Aparelhos Ortodônticos Removíveis , Técnicas de Movimentação Dentária , Adulto , Feminino , Humanos , Masculino , Má Oclusão , Má Oclusão Classe II de Angle/diagnóstico por imagem , Má Oclusão Classe II de Angle/terapia , Maxila/diagnóstico por imagem , Dente Molar/diagnóstico por imagem , Articulação Temporomandibular/diagnóstico por imagem , Técnicas de Movimentação Dentária/métodos , Tomografia Computadorizada de Feixe Cônico
15.
Methods Mol Biol ; 2677: 203-219, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37464244

RESUMO

Recent advances in tissue clearing methodologies have enabled three-dimensional (3D) visualization of the ovary and, consequently, in-depth exploration of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.


Assuntos
Imageamento Tridimensional , Ovário , Feminino , Camundongos , Animais , Imageamento Tridimensional/métodos , Imunofluorescência , Envelhecimento
16.
Lasers Med Sci ; 38(1): 158, 2023 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-37421518

RESUMO

Nd:YAG laser is in common clinical use for the treatment of tissue incision, transpiration, and haemostasis in soft tissues. However, few studies have reported the effects of low-level laser therapy (LLLT) from Nd:YAG laser on bone healing. The aim of this study was to perform three-dimensional (3D) morphological evaluation of the photobiomodulation of Nd:YAG laser in bone defects in rat tibiae using micro-computed tomography CT (micro-CT) imaging. A bone defect was created in each tibia of 30 rats. The right side was treated with LLLT from Nd:YAG laser (LT group) daily until sacrifice and the left tibiae served as controls (control group). All tibiae underwent micro-CT imaging at 7, 14, and 21 days after the operation. Three-dimensional image analysis of bone volume (BV) and bone surface area (BS) of new bone formation in the defects was performed and histologic analysis was conducted for all tibiae. Tibial BV and BS values were highest in both groups at 7 days after the operation and decreased at 14 days after operation. BV and BS values were both significantly higher in the LT group than in the control group at 7 and 14 days. There was no significant difference between the groups for either metric at 21 days. The present findings demonstrate that Nd:YAG laser simulates bone formation during the early healing period.


Assuntos
Lasers de Estado Sólido , Osteogênese , Ratos , Animais , Lasers de Estado Sólido/uso terapêutico , Microtomografia por Raio-X , Tíbia/diagnóstico por imagem , Tíbia/cirurgia , Tíbia/patologia , Imageamento Tridimensional
17.
J Esthet Restor Dent ; 35(8): 1264-1270, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-37317633

RESUMO

STATEMENT OF PROBLEM: Emerging evidence indicates that the emergence profile and subsequent restorative angle play a crucial role in clinical outcomes and can potentially affect the development and progression of peri-implant diseases. However, the traditional evaluation of the emergence profile and angle has been limited to mesial and distal sites using periapical x-rays, without considering the buccal sites. PURPOSE: To describe a novel 3D method to estimate the emergence profile and restorative angles around single implant-supported crowns including buccal sites. MATERIALS AND METHODS: A total of 30 implant-supported crowns (11 molars, 8 premolars, 8 central incisors and 1 canine) were extra-orally scanned using an intraoral scanner and the STL files produced were imported into a 3D software. The crown/abutment interface of each crown was delineated, and apico-coronal lines were automatically drawn following the shape of the crown. Three reference points were defined on the apico-coronal lines at the transition edge of the biological (BC) and the esthetic zone (EC) and the resulting angles were then calculated. The reliability of the measurements (2D and 3D) were assessed using the intraclass correlation coefficient (ICC). RESULTS: In anterior restorations, the mean angle of the esthetic zone amounted to 162 ± 14° at mesial sites, to 140 ± 10° at buccal sites and to 163 ± 11° at distal sites. The corresponding angles at the biological zones, amounted to 155 ± 13° at mesial sites, 139 ± 15° at buccal sites and 157 ± 5° at distal sites. In posterior restorations, the mean angle of the esthetic zone amounted to 162 ± 12° at mesial sites, to 157 ± 13 at buccal sites and to 162 ± 11 at distal sites. The corresponding angles at the biological zone, amounted to 158 ± 8 at mesial sites, 150 ± 15° at buccal sites and 156 ± 10 at distal sites. The ICC for all measurements ranged between 0.77 and 0.99 indicating a good intra-examiner reliability. CONCLUSION: Within the limitations of the present study, the 3D analysis seems to be a reliable and applicable method for the quantitative evaluation of the emergence profile in daily practice. Future randomized clinical trials are needed to assess whether a 3D analysis with the ensuing the emergence profile serves as a predictor for clinical outcomes. CLINICAL SIGNIFICANCE: The development and implementation of a 3D workflow will provide technicians and dentists with the ability to assess the restorative angle of implant-supported restorations during the provisional phase and the final restoration. This approach may help achieve an aesthetically pleasing restoration while minimizing potential clinical complications.


Assuntos
Implantes Dentários para Um Único Dente , Reprodutibilidade dos Testes , Coroas , Dente Pré-Molar , Estética Dentária , Prótese Dentária Fixada por Implante
18.
Front Surg ; 10: 1145059, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37377672

RESUMO

Purpose: With advancements in minimally invasive techniques, oblique lumbar interbody fusion (OLIF) has gained widespread acceptance and is now commonly performed for adult degenerative scoliosis (ADS). The objective of this research paper is to evaluate three-dimensional (3D) intervertebral motions in EOS models before and after surgery and subsequently assess the efficacy of the 3D correction achieved through staged OLIF. Methods: In this retrospective study, 29 consecutive patients diagnosed with ADS were included, with a mean age of 63.6 years, who underwent staged OLIF surgery between 2018 and 2021. Spinopelvic parameters were assessed using EOS images, and 3D models were reconstructed to measure intervertebral motion angles (IMAs) in 70 surgical intervertebral segments, comprising wedge, lordosis, and axial rotation angles. Regression analysis was conducted to compare IMAs in different planes before and after the staged OLIF surgery. Results: Significant three-dimensional correction was observed in 70 intervertebral segments following the first-stage OLIF. The wedge angles decreased from 5.2°± 4.2° to 2.7°± 2.4° (P < 0.001). The lordosis angles increased from 5.1°± 5.9° to 7.8°± 4.6° (P = 0.014), while the axial rotation angles decreased from 3.8°± 2.6° to 2.3°± 2.1° (P < 0.001). Linear regression analysis revealed a positive correlation between wedge angles and axial angles preoperatively (P < 0.001, r = 0.43), as well as between corrected wedge angles and corrected axial angles (P < 0.001, r = 0.42). Conclusion: This study demonstrated that intervertebral motions had a correlation between coronal and axial planes in lumbar degenerative scoliosis. First-stage OLIF was efficient at correcting segmental scoliosis by inserting cages while correcting rotation deformity simultaneously, as well as improving the sagittal spinopelvic parameters.

19.
J Plast Reconstr Aesthet Surg ; 82: 130-136, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-37163828

RESUMO

OBJECTIVE: Chest deformity is one of the complications that occurs after costal cartilage harvesting for auricle reconstruction. In this study, we presented a novel method of free dermofat grafting to repair cartilage defect and aimed to evaluate its effect in ameliorating chest deformity. METHODS: Seventy-six pediatric patients were included in the study, comprising free dermofat grafting group (n = 38) and control group (n = 38). After harvesting costal cartilage, empty perichondrial space of right seventh costal cartilage was filled with free dermofat grafts in free dermofat grafting group. Thoracic computed tomography (CT) was performed three months after surgery and 3D colormap quantification was performed to quantify chest surface asymmetry. The quantified data were further analyzed to compare chest asymmetry level in free dermofat grafting and control groups. RESULTS: In the free dermofat grafting group, the mean of asymmetry level was 2.2 mm. While in the control group, the mean of asymmetry was 5.7 mm. After a comparison between the two groups, the level of asymmetry showed a significant difference (p < 0.01). CONCLUSION: Free dermofat grafting method is easy to perform and a feasible option for ameliorating chest deformity in microtia reconstruction.


Assuntos
Microtia Congênita , Cartilagem Costal , Humanos , Criança , Microtia Congênita/cirurgia , Coleta de Tecidos e Órgãos , Cartilagem Costal/transplante , Orelha Externa/cirurgia , Cartilagem/transplante
20.
Transl Cancer Res ; 12(4): 765-773, 2023 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-37180668

RESUMO

Background: We invest computed tomography (CT) image differences between non-invasive adenocarcinomas (NIAs) and invasive adenocarcinomas (IAs) presenting as pure ground glass nodules (GGNs). Methods: From 2013 to 2019, 48 pure GGNs were surgically resected in 45 patients. Of these, 40 were pathologically diagnosed as non-small cell lung cancers (NSCLCs). We assessed them using the Synapse Vincent (Fujifilm Co., Ltd., Tokyo, Japan) three-dimensional (3D) analysis system; we drew histograms of the CT densities. We calculated the maximum, minimum, means, and standard deviations of the densities. The proportions of GGNs of high CT density were compared between the two groups. The diagnostic performance was investigated via receiver operating curve (ROC) analysis. Results: Of the 40 pure GGNs, 20 were NIAs (4 adenocarcinomas in situ and 16 minimally IAs) and 20 IAs. Significant correlations were evident between histological invasiveness and the maximum and mean CT densities and the standard deviation. Neither the nodule volume nor the minimum CT density significantly predicted invasiveness. A CT volume density proportion >-300 Hounsfield units optimally predicted the invasiveness of pure GGNs; the cutoff was 5.41% with a sensitivity of 85% and a specificity of 95%. Conclusions: CT density reflected the invasiveness of pure GGNs. A CT volume proportion density >-300 Hounsfield units may significantly predict histological invasiveness.

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